Generator
Part:BBa_K2055425:Design
Designed by: Martha Carolina Elizondo Cantú Group: iGEM16_Tec-Monterrey (2016-09-29)
Iron oxidase generator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
It should be used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase (DE3 systems).
Source
The sequence of the iron oxidase was obtained from UniProt. It corresponds to the IRO gene of Acidithiobacillus ferrooxidans, accession number P50500. The UniProt Consortium. UniProt: a hub for protein information. Nucleic Acids Res. 43: D204-D212 (2015)